Molecular Testing Lab

Created on:2020-03-18 11:07

 

 

 

Molecular Testing Lab is a  state-of-the-art, esoteric laboratory, which mainly includes six groups:Sequencing Group, Next-Generation Sequencing Group, Real-time Quantitive PCR Group, Luminex Group, DNA mass spectromety array Group, Affymetrix Group. We specialize in high-complexity molecular testing with an extensive portfolio of services including infectious disease, toxicology, targeted sequencing, single nucleotide polymorphism (SNP) genotyping, Next- Generation Sequencing (NGS), high-resolution immune profiling, and cutting-edge environmental testing. Molecular testing lab offers a variety of clinical trial services comprising of assay development, trial site training, investigator support, and customized reporting.

 

 Group introduction:

 

    Sequencing Group

    Next-Generation Sequencing Group 

    Real-time Quantitative PCR Group

    Luminex Group

    DNA mass spectrometry array Group

   Affymetrix Group

Main tests in Molecular Testing Lab

Test  Name

Clinical Information

Specimen   Type 

Method   Description

Specimen   Requirement

WT1 gene quantitative test

Monitoring response   to therapy in patients with AML or MDS

Bone Marrow or Whole  Blood

Real-Time Polymerase  Chain Reaction (PCR),quantitative PCR
     
   

Container/Tube   Preferred: EDTA (lavender top)

Specimen Volume: 3-5 mL(4℃)

Leukemia fusion gene screening qualitative test

Diagnosis and   prognosis of leukemia

Bone Marrow 

Polymerase Chain   Reaction (PCR),qualitative PCR

Container/Tube   Preferred: EDTA (lavender top)
Specimen Volume: 3-5 mL(4℃)

BCR/ABL1 (CML) quantitative test

Monitoring response   to therapy in patients with chronic myeloid leukemia who are known to have   the e13/a2 or e14/a2 BCR/ABL1 fusion transcript forms

Bone Marrow or Whole   Blood

Real-Time Polymerase Chain Reaction (PCR),quantitative PCR
     
   

Container/Tube   Preferred: EDTA (lavender top)
Specimen Volume: 3-5 mL(4℃)

JAK2 gene V617 mutation qualitative test

Aiding in the   distinction between a reactive blood cytosis and a chronic myeloproliferative   disorder in bone marrow specimens, blood specimens

Bone Marrow or Whole   Blood

Polymerase Chain Reaction (PCR), qualitative PCR

Container/Tube   Preferred: EDTA (lavender top)
Specimen Volume: 3-5 mL(4℃)

PML/RARα quantitative test

Diagnosis of acute   promyelocytic leukemia (APL)
     
    Detection of residual or recurrent APL
     
    Monitoring the level of promyelocytic leukemia/retinoic acid receptor alpha   (PML/RARA) in APL patients

Bone Marrow or Whole   Blood

Real-Time Polymerase Chain Reaction (PCR),quantitative PCR

Container/Tube   Preferred: EDTA (lavender top)
    
Specimen Volume: 3-5 mL(4℃)

BCR/ABL1(ALL) quantitative test

Monitoring response   to therapy in patients with known e1/a2 bcr/abl (p190) fusion forms

Bone Marrow or Whole   Blood

Real-Time Polymerase Chain Reaction (PCR),quantitative PCR

Container/Tube   Preferred: EDTA (lavender top)
    
Specimen Volume: 3-5 mL(4℃)

Epstein-Barr Virus(EBV) DNA quantitative test

A prospective and   diagnostic marker for the development of posttransplant lymphoproliferative   disorders (PTLD), especially in Epstein-Barr virus (EBV)-seronegative organ   transplant recipients who receive anti-lymphocyte globulin for induction   immunosuppression and OKT-3 treatment for early rejection

Whole blood or serum

Real-Time Polymerase   Chain Reaction (PCR),quantitative PCR

Specimen Type:   serum
    Container/Tube: ACD (yellow top)
    Specimen Volume: 3-5 mL(4℃)
    Collection Instructions:Centrifuge
    Specimen Type: Whole blood
    Container/Tube: Lavender top (EDTA) 
    Specimen Volume: 3-5 mL(4℃)

Immunoglobulin gene rearrangement test

Determining whether a   B-cell or plasma cell population is polyclonal or monoclonal in peripheral   blood specimens
     
    Identifying neoplastic cells as having B-cell or plasma cell   differentiation
     
    Monitoring for a persistent neoplasm by detecting an immunoglobulin gene   rearrangement profile similar to one from a previous neoplastic specimen

Bone Marrow or Whole   Blood

Polymerase Chain Reaction (PCR),qualitative PCR

Container/Tube   Preferred: EDTA (lavender top)
    
    Specimen Volume: 3-5 mL(4℃)

T-Cell Receptor gene rearrangement test

Determining whether a   T-cell population is polyclonal or monoclonal

Bone Marrow or Whole   Blood

Polymerase Chain Reaction (PCR),qualitative PCR

Container/Tube   Preferred: EDTA (lavender top)
    
    Specimen Volume: 3-5 mL(4℃)

Cytomegalovirus(CMV) DNA quantitative test

Detection and   quantification of cytomegalovirus (CMV) viremia
     
Monitoring CMV disease progression and response to antiviral therapy

Whole blood or serum

Real-Time Polymerase Chain Reaction (PCR),quantitative PCR

Specimen Type:   serum
    Container/Tube: ACD (yellow top)
    Specimen Volume: 3-5 mL(4℃)
    Collection Instructions:Centrifuge
    Specimen Type: Whole blood
    Container/Tube: Lavender top (EDTA) 
    Specimen Volume: 3-5 mL(4℃)

Jak2 gene V617 mutation quantitative

Aiding in the   distinction between a reactive cytosis and a chronic myeloproliferative   disorder

Bone Marrow or Whole   Blood

Real-Time Polymerase Chain Reaction (PCR),quantitative PCR

Container/Tube   Preferred: EDTA (lavender top)
    
    Specimen Volume: 3-5 mL(4℃)

FLT3/ITD gene mutation test

A prognostic   indicator in some acute myeloid leukemia patients

Bone Marrow or Whole   Blood

Polymerase Chain Reaction (PCR)/ Sanger Sequencing

Container/Tube   Preferred: EDTA (lavender top)
    
    Specimen Volume: 3-5 mL(4℃)

ABL1 tyrosine kinase region mutation test

Detection of Imatinib   Resistance

Bone Marrow or Whole   Blood

Polymerase Chain Reaction (PCR)/ Sanger Sequencing

Container/Tube   Preferred: EDTA (lavender top)
    
    Specimen Volume: 3-5 mL(4℃)

CALR gene exon 9 mutation test

Aiding in the   distinction between a reactive cytosis and a chronic myeloproliferative   disorder
     
    Evaluates for mutations in CALR in an algorithmic process for the MPNR /   Myeloproliverative Neoplasm (MPN), JAK2 V617F with reflex to CALR and MPL

Bone Marrow or Whole   Blood

Polymerase Chain Reaction (PCR)/ Sanger Sequencing

Container/Tube   Preferred: EDTA (lavender top)
    
    Specimen Volume: 3-5 mL(4℃)

C-kit/D816V gene mutation test

Diagnosing systemic mastocytosis 

Bone Marrow or Whole   Blood

Polymerase Chain Reaction (PCR)/ Sanger Sequencing

Container/Tube   Preferred: EDTA (lavender top)
    
    Specimen Volume: 3-5 mL(4℃)

CEBPA gene mutation test

Initial evaluation of   acute myeloid leukemia, both for assigning an appropriate diagnostic subclassification and as an aid for determining prognosis

Bone Marrow or Whole   Blood

Polymerase Chain   Reaction (PCR)/ Sanger Sequencing

Container/Tube   Preferred: EDTA (lavender top)
    
    Specimen Volume: 3-5 mL(4℃)

NPM1 gene mutation  test

As a prognostic   indicator in patients with newly diagnosed acute myelogenous leukemia with normal karyotype and no FLT3 mutation

Bone Marrow or Whole   Blood

Polymerase Chain Reaction (PCR)/ Sanger Sequencing

Container/Tube   Preferred: EDTA (lavender top)
    
    Specimen Volume: 3-5 mL(4℃)

AML1/ETO fusion gene quantitative test

As a prognostic   indicator in patients

Bone Marrow or Whole   Blood

Real-Time Polymerase Chain Reaction (PCR),quantitative PCR

Container/Tube   Preferred: EDTA (lavender top)
    
    Specimen Volume: 3-5 mL(4℃)

MPL gene exon 10 mutation test

Aiding in the   distinction between a reactive cytosis and a myeloproliferative neoplasm when   JAK2V617F testing result is negative.
     
Evaluates for mutations in MPL in an algorithmic process for the MPNCM / MPN (CALR, MPL) Reflex.

Bone Marrow or Whole   Blood

Polymerase Chain Reaction (PCR)/ Sanger Sequencing

Container/Tube   Preferred: EDTA (lavender top)
    
    Specimen Volume: 3-5 mL(4℃)

Post transplantation Donor Chimerism test 

Determining the   relative amounts of donor and recipient cells in a specimen in sorted cell fractions
     
An indicator of bone marrow transplant success

Bone Marrow or Whole   Blood

Polymerase Chain Reaction (PCR) Amplification/Capillary Electrophoresis

Container/Tube   Preferred: EDTA (lavender top)
    
    Specimen Volume: 3-5 mL(4℃)