Clinical Immunology Lab

Created on:2020-03-18 11:30

There is automatic detection equipment such as automatic western blotting instrument, automatic indirect immunofluorescence analyzer, automatic chemiluminescence detector and automatic quantitative allergen detector. Screening and qualitative and quantitative detection of various autoantibodies are developed, and the reports were well illustrated. In addition, tests for inhaled and ingested allergens and various combinations of food intolerance tests are newly introduced.

In collaboration with Immun-array, USA, the early screening of systemic lupus erythematosus - the performance verification of the SLE-key project wascarried out in Autoimmunity group.

 

Instruments and apparatus 

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Roche Cobsa 6000 system (combination of Cobas e601 and Roche c501)

 

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Siemens - IMMULITE 2000 automatic chemiluminescence instrument

 

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Siemens -Advia Centaur XP automated chemiluminescence immunoassay analyzer

 

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Siemens-BN prospec-automatic specific protein analyzer

Main tests in Immunology Lab

Test  Name

Clinical Information

Specimen  Type 

Method   Description

Specimen   Requirement

1,3- beta –D Glucan   test(G test)

The Fungitell Beta –   D Glucan assay detects (1,3) – Beta-D-glucan from the following pathogens:   Candida spp., Acremonium, Aspergillus spp., Coccidioides immitis, Fusarium   spp., Histoplasma capsulatum, Trichosporon spp., Sporothrix schenckii,   Saccharomyces cerevisiae, and Pneumocystis jiroveci.

Whole Blood、Serum、Lavage

Nephelometry

Specimen Type: Whole   Blood、Serum
    Container/Tube Preferred: Sterile G test special
    Specimen Volume: 4mL(4℃)

Galactomannan test(GM test)

An aid in the   diagnosis of invasive fungal and assessing response to therapy

Whole Blood、Serum、Lavage

ELISA

Container/Tube   Preferred: Serum gel
    Acceptable: Red top or yellow top
    Specimen Volume: 4mL(4℃)
    Collection Instructions:
    1. Avoid exposure of specimen to atmosphere.
    2. Send specimen in original tube. Do not aliquot.

FLC(free light chains) Kappa and Lambda test

Monitoring patients   with monoclonal light chain diseases 

Serum、Urine

Nephelometry

Specimen   Type:Serum
    Container/Tube Preferred: Serum gel
    Acceptable: Red top
    Specimen Volume: 3 mL(4℃)
    Specimen Type:Urine
    Container/Tube Preferred: No antiseptic
    Specimen Volume: 100 mL(4℃)

EPO(erythropoietin) test 

An aid in   distinguishing between primary and secondary polycythemia
     
    Differentiating between appropriate secondary polycythemia (eg,   high-altitude living, pulmonary disease, tobacco use) and inappropriate   secondary polycythemia (eg, tumors)
     
    Identifying candidates for erythropoietin (EPO) replacement therapy (eg,   chronic renal failure)
     
    Evaluating patients undergoing EPO replacement therapy who demonstrate an   inadequate hematopoietic response

Serum

Immunoenzymatic Assay

Container/TubePreferred:   Serum gel
    Acceptable: Red top
    Specimen Volume: 2 mL(4℃)

Whole set of electrophoresis 1(Serum Protein Electrophoresis, Whole   blood immune fixation electrophoresis, Piss immune fixation, Total protein   test, Albumin, Albumin/Globulin ration, IgA/G/M quantitative test, Piss/Blood   light chain Kappa and Lambda)

An aid in the   diagnosis of multiple myeloma

serum and urine

Agarose Gel   Electrophoresis, Turbidimetric ImmunoAssay

Specimen   Type:Serum
    Container/Tube Preferred: Serum gel
    Acceptable: Red top
    Specimen Volume: 3 mL(4℃)
    Specimen Type:Urine
    Container/Tube Preferred: No antiseptic
    Specimen Volume: 100 mL(4℃)

Whole set of Electrophoresis 2(Serum Protein Electrophoresis, Whole   blood immune fixation electrophoresis, urine immune fixation, Total protein   test, Albumin, Albumin/Globulin ration, IgA/G/M/D/E quantitative test,   Piss/Blood light chain Kappa and Lambda)

An aid in the   diagnosis of multiple myeloma

serum and urine

Agarose Gel   Electrophoresis, Turbidimetric ImmunoAssay

Specimen   Type:Serum
    Container/Tube Preferred: Serum gel
    Acceptable: Red top
    Specimen Volume: 3 mL(4℃)
    Specimen Type:Urine
    Container/Tube Preferred: No antiseptic
    Specimen Volume: 100mL(4℃)

Cyclosporine test

Monitoring whole   blood cyclosporine concentration during therapy, particularly in individuals   coadministered CYP3A4 substrates, inhibitors, or inducers
     
    Adjusting dose to optimize immunosuppression while minimizing   toxicity
     
    Evaluating patient compliance

Whole Blood 

High-Performance   Liquid Chromatography-Tandem Mass Spectrometry (HPLC-MS/MS)

Container/Tube   Preferred: EDTA (lavender top)
   
    Specimen Volume: 3-5 mL(4℃)

TB-IGRA test

Indirect test for   Mycobacterium tuberculosis complex infection (latent tuberculosis infection).   This test should not be used for diagnosis of active tuberculosis (TB)   infection.
     
    This test can be used as an aid to detect latent TB infection.

Whole blood

Enzyme-Linked   Immunosorbent Assay (ELISA)

Container/Tube: Green   top (Heparin lithium)
    Specimen Volume: 4 mL

Antinuclear antibody ANA

Evaluating patients   suspected of having a systemic rheumatic disease

Serum

Indirect   immunofluoresent assay, IIF

Container/Tube:
    Preferred: Serum gel
    Acceptable: Red top
    Specimen Volume: 2 mL(4℃)

Whole set of EBV test(Anti-EB virus capsid antigen IgA/G/M、Anti-EB virus nuclear antigen IgG、Anti-EB viral early antigen IgG)

Diagnosing infectious   mononucleosis when a mononucleosis screening procedure is negative and   infectious mononucleosis or a complication of Epstein-Barr virus infection is   suspected

Serum

ELISA

Container/Tube:
    Preferred: Serum gel
    Acceptable: Red top
    Specimen Volume: 2 mL(4℃)

Immunoglobulin E/A/G/M test

Evaluation of   patients with suspected diseases associated with elevations in total   immunoglobulin E (IgE), including allergic disease, primary   immunodeficiencies, infections, malignancies, or other inflammatory   diseases
     
    Diagnostic evaluation of patients with suspected allergic bronchopulmonary   aspergillosis
     
    Identification of candidates for omalizumab (anti-IgE) therapy

Serum

immunonephelometry

Container/Tube:
    Preferred: Serum gel
    Acceptable: Red top
    Specimen Volume: 2 mL(4℃)

CMV (cytomegalovirus) IgM test

Diagnosis of primary,   acute phase infection with cytomegalovirus (CMV), especially in patients with   infectious mononucleosis and pregnant women who, based on clinical signs or   exposure, may have primary CMV infection

Serum

ELISA

Container/Tube:
    Preferred: Serum gel
    Acceptable: Red top
    Specimen Volume: 2 mL(4℃)

Antihuman globulin test (coombs’)

Demonstrating in vivo   coating of RBCs with IgG or the complement component C3d in the following   settings:
    -Autoimmune hemolytic anemia
    -Hemolytic transfusion reactions
    -Drug-induced hemolytic anemia

Whole Blood and Serum

Direct Antiglobulin   Test/Standard AABB

Container/Tube: Green   top (Heparin lithium)
    Specimen Volume: 4 mL(4℃)
    Container/Tube Preferred: Serum gel
    Acceptable: Red top
    Specimen Volume: 2 mL(4℃)

Antibodies to Extractable Nuclear Antigens test (7 item:
    1:Anti-RNP (Anti-Ribonucleoprotein)
    2:Anti-Smith (SM) Antigen
    3-4:Anti-SSA (Anti-Sjogren's Syndrome A(60kd/52kd))
    5:Anti-SSB (Anti-Sjogren's Syndrome B)
    6:DNA Topoisomerase 1 Antibodies(Scl 70 Antibodies)
    7:Histidyl-tRNA Synthetase Antibodies(Jo 1 Antibodies)

Evaluating patients   with signs and symptoms of a connective tissue disease in whom the test for   antinuclear antibodies is positive

Serum

ELISA

Container/Tube:
    Preferred: Serum gel
    Acceptable: Red top
    Specimen Volume: 2 mL(4℃)

RF-IgA/G/M test

Diagnosis and   prognosis of rheumatoid arthritis

Serum

ELISA

Container/Tube:
    Preferred: Serum gel
    Acceptable: Red top
    Specimen Volume: 2 mL(4℃)

Anti-B2-GPI test

Evaluation of   patients with suspected antiphospholipid syndrome by identification of beta-2   GP1 IgM antibodies

Serum

ELISA

Container/Tube:
    Preferred: Serum gel
    Acceptable: Red top
    Specimen Volume: 2 mL(4℃)

Ferritin test

Aiding in the   diagnosis of iron deficiency and iron overload conditions
     
    Differentiating iron deficiency anemia and anemia of chronic disease

Serum

Immunoenzymatic Assay

Container/Tube:
    Preferred: Serum gel
    Acceptable: Red top
    Specimen Volume: 2 mL(4℃)

ACL(anticardiolipin antibody) test IgG Ig M Ig A

Evaluation of   patients with suspected antiphospholipid syndrome by identification of   phospholipid IgG antibodies

Serum

Enzyme-Linked   Immunosorbent Assay (ELISA)

Container/Tube:
    Preferred: Serum gel
    Acceptable: Red top
    Specimen Volume: 2 mL(4℃)

Vitamin B12 test

Investigation of   macrocytic anemia
     
    Workup of deficiencies seen in megaloblastic anemias

Serum

Immunoenzymatic Assay

Container/Tube:
    Preferred: Serum gel
    Acceptable: Red top
    Specimen Volume: 2 mL(4℃)

Folic acid test

Investigation of   suspected folate deficiency

Serum

Competitive-Binding   Receptor Assay

Container/Tube:
    Preferred: Serum gel
    Acceptable: Red top
    Specimen Volume: 2 mL(4℃)