Flow Cytometry Lab

Created on:2020-03-18 11:58

Flow Cytometry (FCM) is a multi-parameter, rapid analysis and sorting technology that is capable of rapidly analyzing and sorting single cells or other biological particles in a state of rapid linear flow at certain functional level. It is one of the most advanced cell analysis techniques. FCM has been widely used in clinical and basic medical researches, such as immunology, hematology, oncology, cell biology, cytogenetics, biochemistry, etc. It can be used for typing of leukemia/lymphoma, tumor cell heteroploid analysis and immunological studies, etc.

 

Instruments and apparatus         

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BD FACSCanto II

The FACSCanto II system has 8-color novel ultra-high sensitivity optical system of three laser standard, and it is the world's most advanced resolution instruments that can improve the resolution of rare samples and weakly positive samples; its powerful digital electronic system can provide more accurate experiment data, and enhance the ability of high-throughput assays during screening.  

 

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BD FACSCalibur

The BD FACSCalibur™ platform allows users to perform both cell analysis and cell sorting in a single benchtop system. The system supports a wide variety of research and clinical applications and is complemented by a broad suite of intuitive software solutions to streamline analysis for a wide range of applications including enumeration of lymphocyte subsets, stem cells, residual white blood cells, and reticulocytes.

 

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Guava® easyCyte Flow Cytometer

Guava easyCyte series micro-capillary cell analysis platform: The micro-capillary patent flow design, the construction of the ultra-micro experimental system and the new thinking of the “one drop for flow” experimental design, make it possible to carry out flow detection of precious clinical and research specimens.

Main tests in Flow Cytometry Lab

Items        

Specimen Requirements

Methods

Clinical Significance

Turn Around Time

Immunophenotyping

2-3 ml of marrow/peripheral blood (the percentage of abnormal cells in peripheral blood >20%), EDTA anticoagulation tube, 100 mg of fresh active tissues, soaked and stored in 1640 medium or normal saline, 200 ml of pleural effusion and ascites stored in a sterile bottle

Flow cytometry

Valuable for the diagnosis, typing, development of therapeutic schedules and prognosis of malignant hematological diseases

 

 

 

 

 

Monday to Friday; 3 working days

 

 

Lymphoma immunophenotyping

Valuable for the diagnosis, typing, development of therapeutic schedules and prognosis of lymphoma diseases

Full package of lymphoid immunophenotyping

6-8ml of bone marrow, EDTA anticoagulation tube

Valuable for the diagnosis of acute lymphocytic leukemia (ALL), nearly 100 antibodies used at a time, achieving more complete and accurate diagnosis

 

 

 

 

 

Monday to Friday; 5 working days

 

 

 Full package screening of lymphoid immunophenotyping

Auxiliary diagnosis of leukemia, nearly 100 antibodies used at a time

Dynamic monitoring of minimal residual focus of B-ALL

3ml of bone marrow, EDTA anticoagulation tube, the patient ID number must be provided for each test (no ID number, no dynamic monitoring report can be made)

Dynamic monitoring of B-ALL MRD

Detection of minimal residual leukemia

2-3ml of bone marrow, EDTA anticoagulation tube, please fill in the application form, and attach the immunophenotyping data at first visit

Evaluation of the efficacy and prognosis of leukemia (ALL, AML, etc.), and helpful for the earlier prediction of the recurrence of leukemia

Monday to Friday; 3-5 working days

Lymphocyte subsets

3ml of peripheral blood, EDTA anticoagulation tube

Detection of T, B, NK lymphocyte subsets, monitoring and reflection of the patient’s immune function

Monday to Friday; 2 working days

DNA ploidy detection

Fresh tissues/exfoliative cells, stored in normal saline, and marked; body fluids: pleural effusion, pericardial effusion, cerebrospinal fluid (stored in sterile tubes)

Evaluation of cell cycle distribution and analysis of DNA ploidy, based on which early diagnosis of tumors, the benign and malignant borderline tumors and mesenchymal tumors can be determined

Monday to Saturday; 3 working days

 

PNH-FLAER detection (CD45 CD14 CD33 CD24 Flaer)

2-3ml of peripheral blood, EDTA anticoagulation tube

Valuable for the diagnosis of disease progression and efficacy in PNH patients; for patients with hemocytopenia under long-term immunosuppressive therapy, especially for AA, MDS and other diseases, monitoring of clonal changes and early detection of changes can help differentiate from patients with partial immune cytopenia.

Monday to Friday; 3 working days

Detection of minimal residual lesion-related antigen in neuroblastoma

2-3ml of bone marrow, EDTA anticoagulation tube, 4°C, delivered for testing within 72h

Valuable for early detection of residual neuroblastoma lesions, providing information for the selection of clinical therapeutic schedules and prognosis

Monday to Friday; 3-5 working days

Platelet membrane surface antibody A

6ml of peripheral blood, sodium citrate coagulation tube (1:9) or EDTA anticoagulation tube, (please attach detailed clinical diagnosis and auxiliary test results)

One of the important diagnostic indexes of idiopathic thrombocytopenic purpura (ITP)

Monday to Sunday; 3 working days

 
 
 

Platelet membrane surface antibody B

 

NK cell lymphoma/leukemia-related antigens detection

1. 2-3ml of bone marrow or peripheral blood, EDTA anticoagulation tube; 2. Solid tumor specimens such as ymph nodes are placed in a sterile bottle with normal saline; 3. If the specimens are pleural effusion and ascites, they should be placed in a clean container

Assist in the identification and diagnosis of NK cell lymphoma/leukemia

Monday to Friday; 3-5 working days

*Th1/Th2 detection

3ml of peripheral blood, heparin anticoagulation tube

Valuable for understanding immune balance, evaluating disease progression, and designing clinical protocols

Monday to Friday; 3 working days

MM minimal residue detection

2ml of bone marrow, EDTA anticoagulation tube

With respect to the presence of normal and abnormal plasma cells in the patient’s bone marrow, a multi-parameter, multi-azimuth analysis through FACS Canto II (8-color flow cytometry) method is performed by using one-tube method to accurately find out abnormal plasma cells to improve accuracy after MM treatment

Monday to Sunday; 5 working days

CD34+ hematopoietic stem cell count

3ml of bone marrow, EDTA anticoagulation tube, 4°C, delivered for testing within 72h

Determination of the best time for stem cell transplantation and provision of a reference for bone marrow transplantation

Monday to Friday; 3 working days