Cytogenetic Lab

Created on:2020-03-18 12:25

Cytogenetic analysis is one of the basic contents of MICM typing of hematologic malignancies recommended by WHO. Many specific chromosomal aberrations are associated with specific hematologic subtypes, and therefore they become important indicators of the diagnostic typing of hematologic malignancies. The chromosomal karyotype has an independent prognostic value in the diagnosis of hematologic malignancies.   

Florescence in Situ Hybridization (FISH) is a detection technique of in situ hybridization of samples using non-radioactive fluorescent signals. The technique is easy to operate for quick detection, with high repeatability and specificity, and it can complement with the karyotype analysis.

 

Instruments and apparatus  

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HANABI PIIIplus Chromosome Harvester

 

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Isis Fluorescence Imaging Platform(ZEISS&MetaSystems)

 

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Metafer- Automated chromosome Scanning Platform(ZEISS&MetaSystems)

 

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Leica CytoVision GSL-120 high throughput automatic chromosome scanning platform

 

Main tests in Cytogenetic  Lab

 

Test Name

Clinical Information 

Specimen   Type 

Method   Description

Specimen   Requirement

TP53 FISH test

Aids in the   identification of neoplastic cells

Bone Marrow or Whole   Blood 

Fluorescence In Situ Hybridization (FISH)

Container/Tube Preferred:Heparin
 Specimen Volume: 3 -5mL(4℃)

IGH/CCND1 FISH test

Diagnosis and   prognosis of lymphoma and multiple myeloma、Chronic lymphocytic leukemia

Bone Marrow or Whole   Blood 

Fluorescence In Situ Hybridization (FISH)

Container/Tube   Preferred:Heparin
Specimen Volume: 3 -5mL(4℃)

x/y Gene FISH test

Identifying the sex chromosome  complement

Bone Marrow or Whole   Blood 

Fluorescence In Situ   Hybridization (FISH)

Container/Tube   Preferred:Heparin
Specimen Volume: 3 -5mL(4℃)

1q21 FISH test

Diagnosis and   prognosis of multiple myeloma

Bone Marrow or Whole   Blood 

Fluorescence In Situ   Hybridization (FISH)

Container/Tube   Preferred:Heparin
Specimen Volume: 3 -5mL(4℃)

IGH/FGFR-3 FISH test

Aiding in the   diagnosis, phenotyping and prognosis of multiple myeloma

Bone Marrow or Whole   Blood

Fluorescence In Situ   Hybridization (FISH)

Container/Tube   Preferred:Heparin
Specimen Volume: 3 -5mL(4℃)

IGH/MAF FISH test

Aiding in the   diagnosis, phenotyping and prognosis of multiple myeloma

Bone Marrow or Whole   Blood

Fluorescence In Situ   Hybridization (FISH)

Container/Tube   Preferred:Heparin
Specimen Volume: 3 -5mL(4℃)

7q- FISH test

A prognostic   indicator in myelodysplastic syndrome

Bone Marrow or Whole   Blood

Fluorescence In Situ   Hybridization (FISH)

Container/Tube   Preferred:Heparin
Specimen Volume: 3 -5mL(4℃)

BCR/ABL FISH test

Monitoring response   to therapy in patients with chronic myeloid leukemia who are known to have   the e13/a2 or e14/a2 BCR/ABL1 fusion transcript forms

Bone Marrow or Whole   Blood

Fluorescence In Situ   Hybridization (FISH)

Container/Tube   Preferred:Heparin
Specimen Volume: 3 -5mL(4℃)

5q- FISH test

A prognostic   indicator in myelodysplastic syndrome

Bone Marrow or Whole   Blood

Fluorescence In Situ   Hybridization (FISH)

Container/Tube   Preferred:Heparin
Specimen Volume: 3 -5mL(4℃)

CEP8 FISH test

A prognostic   indicator in myelodysplastic syndrome

Bone Marrow or Whole   Blood

Fluorescence In Situ   Hybridization (FISH)

Container/Tube   Preferred:Heparin
Specimen Volume: 3 -5mL(4℃)

20q- FISH test

A prognostic   indicator in myelodysplastic syndrome

Bone Marrow or Whole   Blood

Fluorescence In Situ   Hybridization (FISH)

Container/Tube   Preferred:Heparin
Specimen Volume: 3 -5mL(4℃)

19q FISH test

Aids in diagnosing oligodendroglioma tumors and predicting the response of an oligodendroglioma to therapy
   

May be useful in tumors with a complex "hybrid" morphology  requiring differentiation from pure astrocytomas to support the presence of   oligodendroglial differentiation/lineage
     
Indicated when a diagnosis of oligodendroglioma, both low-grade World   Health Organization (WHO, grade II) and anaplastic (WHO, grade III) is   rendered
     
Strongly recommended when a diagnosis of mixed oligoastrocytomas is   rendered

Tissue

Fluorescence In Situ   Hybridization (FISH)

Specimen Type:   Tissue
Preferred: Tissue block
 Collection Instructions: Submit a formalin-fixed, paraffin-embedded (FFPE)   tumor tissue block. Blocks prepared with alternative fixation methods may be   acceptable; provide fixation method used.
     
Acceptable: Slides
Collection Instructions: Six consecutive, unstained, 5 micron-thick   sections placed on positively charged slides, and 1 hematoxylin and   eosin-stained slide.